We have examined the influence of both dietary fish oil and probucol on monocyte adhesion to the aortic endothelium rats fed an atherogenic diet for 2 weeks. All rats were fed a low-fat diet supplemented with 4% cholesterol, 1% cholic acid, and 0.5% 2-thiouracil. In addition to the atherogenic diet, group 1 (FO; n = 20) received a dietary supplement of the fish oil concentrate MaxEPA (5% w/w); group 2 (CO; n = 20) received a supplement of a control oil with same polyunsaturated-monounsaturated-saturated fatty acid ratio as Max-EPA; and group 3 (PR; n = 20) received both the control oil supplement (5% w/w) and a 1% (w/w) supplement of probucol. Analysis of blood samples taken at 2 weeks revealed that both fish oil and probucol lowered total plasma cholesterol by 30% compared with the CO group. In addition, fish oil supplementation caused a significant decrease in cholesterol contained in the VLDL fraction while probucol supplementation caused a significant lowered cholesterol in the HDL fraction. Analysis of mononuclear cell adhesion to the aortic endothelium in vivo revealed that, fish oil had no significant effect probucol reduced adhesion by 40%. The results of this study suggest that probucol, but not fish oil, may inhibit the initiation of lesion formation in the rat model of atherosclerosis.
研究鱼油和普罗布考对致动脉粥样硬化饮食饲养2周的大鼠体内单核细胞与主动脉内皮黏附的影响。大鼠接受添加4%胆固醇，1%胆酸以及2%硫脲嘧啶的低脂饮食饲养。除接受致动脉粥样硬化的饮食饲养外，1组(FO; n = 20)饮食中添加浓缩鱼油脉适宝(5% w/w)；2组(CO; n = 20)饮食中添加与脉适宝多不饱和-单不饱和-饱和脂肪比例相同的对照油；3组(PR; n = 20)饮食中添加对照油(5% w/w)与普罗布考(1% w/w)。2周后血样分析结果显示：与CO组相比，鱼油和普罗布考能够使血浆总胆固醇含量降低30%。鱼油使VLDL胆固醇含量显著降低，普罗布考使HDL胆固醇显著降低。对体内单核细胞与主动脉内皮的黏附分析显示，鱼油对黏附无影响，而普罗布考是黏附降低40%。试验结果表明，普罗布考而非鱼油能够抑制动脉粥样硬化大鼠模型损伤的形成。
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